Separation of rabbit adrenal cells by sedimentation at a linear gradient of albumin concentration.

نویسنده

  • K Abe
چکیده

The isolated cells of rabbit adrenal glands were separated by the method of sedimentation at a linear gradient of albumin concentration and incubated with 14C-cholesterol. The corticosterone and cortisol produced by the separated cells were fractionated by thin-layer chromatography and the radioactivities in these steroids were measured. The more rapidly sedimenting cells showed a greater ratio of cortisol production to corticosterone production than the more slowly sedimenting cells. This phenomenon is not due to the decrease of corticosterone production per cell by the former, but to the increase of cortisol production per cell. These results suggest that the rabbit adrenal cortex may be composed of the cells that produce corticosterone and cortisol in different ratio, i.e., physiologically heterogeneous cells. The functional specificity on different cellular zones of the adrenal cortex in many mammals was confirmed by a number of workers, that is, aldosterone and a part of corticosterone was produced by the zona glomerulosa, and corticosterone and cortisol by both the zona fasciculata and reticularis (GIROUD et al., 1956; STACHENKO and GIROUD, 1959). However, it has not been evident whether corticosterone and cortisol are produced by the same cell or different cells. In an attempt to elucidate this problem, the separation of rabbit adrenal cells was carried out in the present study by the method of sedimentation at a linear gradient of albumin concentration. MATERIALS AND METHODS Isolation of adrenal cells. Bilateral adrenal glands from a 30-40-day-old male rabbit were used to prepare cell suspensions because they produced both corticosterone and cortisol and were easily isolated by the method described below. Received for publication March 13, 1975 安倍 紀一郎

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عنوان ژورنال:
  • The Japanese journal of physiology

دوره 25 5  شماره 

صفحات  -

تاریخ انتشار 1975